Research Area
- Bioanalytical chemistry based on advanced design and synthesis of chemical probes
- Design and synthesis of fluorescent peptide nucleic acids, peptides, small molecules and DNA-based aptamers for targeting biomarkers including non-coding RNA and extracellular vesicles
- Development of highly sensitive and selective detection methods for non-coding RNA and virus RNA
- Development of fluorogenic dyes for RNA imaging in living cells
- Development of RNA-binding fluorescent indicators for high-throughput drug screening
- Development of detection methods for extracellular vesicle
- Development of RNA tag for the imaging of membrane proteins
- Development of fluorogenic probes for hyaluronan
- Development of small ligands for the detection of DNA damage
Our research interests focus on bioanalytical chemistry based on the advanced design and synthesis of chemical probes that target significant biomarkers such as non-coding RNA and extracellular vesicles. We have created novel probes based on peptide nucleic acids, peptides, small organic molecules, or DNA-based aptamers, and have developed a highly sensitive and selective detection or imaging methods for biological research and drug discovery. Our recent progress includes the development of triplex-forming peptide nucleic acid for fluorescence sensing of double-stranded RNA, fluorescent probes for siRNA delivery imaging in living cells, and fluorescent ligands for mircoRNA detection. By providing such chemical probe-based bioanalytical methods, we are trying to contribute to the further development of life science.
Selected Publications
- "Deep-red light-up signaling of benzo[c,d]indole-quinoline monomethine cyanine for imaging of nucleolar RNA in living cells and for sequence-selective RNA analysis"
Y. Yoshino, Y. Sato, S. Nishizawa, Anal. Chem., 91, 14254-14260 (2019). - "Strong binding and off-on signaling functions of deep-red fluorescent TO-PRO-3 for influenza A virus RNA promoter region"
Y. Sato, Y. Aiba, S. Yajima, T. Tanabe, K. Higuchi, S. Nishizawa, ChemBioChem., 20, 2752-2756(2019). - "Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA"
Y. Sato, S. Yajima, A. Taguchi, K. Baba, M. Nakagomi, Y. Aiba, S. Nishizawa, Chem. Commun., 55, 3183–3186 (2019) - "Fluorescent trimethylated naphthyridine derivative with an aminoalkyl side chain as the tightest non-aminoglycoside ligand for the bacterial A-site RNA"
Y. Sato, M. Rokugawa, S. Ito, S. Yajima, H. Sugawara, N. Teramae, S. Nishizawa, Chem. Eur. J., 24, 13862–13870(2018) - "Optimization of the Alkyl Linker of TO Base Surrogate in Triplex-Forming PNA for Enhanced Binding to Double-stranded RNA"
T. Sato, Y. Sato, S. Nishizawa, Chem. Eur. J., 23, 14079–4088 (2017) - "Triplex-forming Peptide Nucleic Acid Having Thiazole Orange as a Base Surrogate for Fluorescence Sensing of Double-stranded RNA"
T. Sato, Y. Sato, S. Nishizawa, J. Am. Chem. Soc., 138, 9397-9400 (2016) - "Synthetic fluorescent probes capable of selective recognition of 3'-overhanging nucleotides for siRNA delivery imaging"
T. Sato, Y. Sato, K. Iwai, S. Kuge, S. Nishizawa, N. Teramae, Chem. Commun., 51, 1421-1424 (2015) - "Abasic Site-Binding Ligands Conjugated with Cyanine Dyes for "Off-On" Fluorescence Sensing of Orphan Nucleobases in DNA Duplexes and DNA/RNA Hybrids"
Y. Sato, M. Kudo, Y. Toriyabe, S. Kuchitsu, C.-X. Wang, S. Nishizawa, N. Teramae, Chem. Commun., 50, 515-517 (2014) - "Strong and Selective Binding of Amiloride to an Abasic Site in RNA Duplexes: Thermodynamic Characterization and MicroRNA Detection"
Y. Sato, T. Ichihashi, S. Nishizawa, N. Teramae, Angew. Chem. Int. Ed., 51, 6369-6372 (2012)