Patent
  • 佐藤雄介、西澤精一、永富良一
    国際出願番号：PCT/JP2021/030716
    国際出願日：2021年8月23日
  • 西澤精一, 佐藤雄介, 芳野幸奈, 富樫奈央, 樋口啓
    名称：RNA検出用蛍光色素及びその使用方法
    特許第7029841号
    登録日：2022年2月24日
  • 佐藤雄介, 西澤精一, 田邊貴昭, 三浦弘真, オケケ シオマ ウシュ, 川口敦史
    名称：ウイルスRNAの複製と転写を阻害する複合体
    出願番号：特願2020-129884
    出願日：2020年7月31日
  • 佐藤雄介, 西澤精一, 田邉貴昭, 川口敦史
    名称：（仮）インフルエンザA型ウイルスRNA複製の制御方法
    出願番号：特願2019-141527
    出願日：2019年7月31日

  2024
  • Akunna F. Ujuagu, Yusuke Sato, En Ting Tabitha Lee, Seiichi Nishizawa
    "Design of deep-red emissive forced intercalation-induced light-up peptide as an indicator for the HIV-1 TAR RNA-ligand assay: Integration of benzo[c,d]indole-quinoline (BIQ) cyanine dye into Tat peptide"
    Anal. Sci., accepted.

  • Masahiro Okada, Yusuke Sato, Toshihiko Ogura, Tetsuji Itoh, Seiichi Nishizawa
    "Immobilization of Liposomes into Porous Anodized Aluminum Oxide for On-demand Release by Ultrasonic Irradiation: Implications for Drug Delivery"
    ACS Appl. Nano Mater., 7(14), 17074-17080 (2024). [DOI:10.1021/acsanm.4c03267] (Highlighted at the Supplementary cover).

  • 佐藤雄介
    "エクソソーム粒子解析を指向した分子プローブの開発"
    膜, 49, 159-163 (2024).

  • En Ting Tabitha Lee, Yusuke Sato, Akunna F. Ujuagu, Seiichi Nishizawa
    "Forced intercalation-induced light-up peptides as fluorogenic indicators for HIV-1 TAR RNA-ligand assay"
    Analyst, 2024, Advanced Article. [DOI:10.1039/D4AN00530A] (Selected as Analyst HOT Articles 2024).

  • Nao Togashi, Masaaki Nagaoka, Kei Higuchi, Yukina Yoshino, Yawen Wu, Yusuke Sato, Seiichi Nishizawa
    "Fine-tuning of highly bright benzo[c,d]indole-oxazolopyridine cyanine dye for nucleolar RNA imaging in living cells"
    Talanta Open, 9, 100308 (2024). [DOI:10.1016/j.talo.2024.100308] (special issue: "Novel Cell Culture and Manipulation Technologies for Analytical Chemistry" Edited by Kiichi Sato and Kae Sato)

  • 西澤精一, 芳野幸奈, 何夢夢, 樋口啓, 富樫奈央, 鈴木理志, 五十嵐友梧, 佐藤雄介
    "モノメチンシアニン蛍光色素による生細胞核小体RNAイメージング"
    分析化学, 73, 79-86(2024). [DOI:10.2116/bunsekikagaku.73.79] （総合論文：バイオ分析研究懇談会特集号）

  • Shinoda Hidetoshi, Higano Raiha, Oizumi Takashi, Nakamura Asako, Kamijo Toshio, Takahashi Mio, Nagaoka Masaaki, Sato Yusuke, Yamaguchi Akira
    "Albumin Hydrogel–Coated Mesoporous Silica Nanoparticle as Carrier of Cationic Porphyrin and Ratiometric Fluorescence pH Sensor"
    ACS Appl. Bio Mater., 7,2, 1204–1213(2024). [DOI:10.1021/acsabm.3c01103].

  • Xinyu Fan, Yusuke Sato, Yudai Shiraki, Seiichi Nishizawa
    "Design of synthetic peptide-based fluorescent probes for turn-on detection of hyaluronan"
    Anal. Sci., 40, 609-614 (2024).[DOI:10.1007/s44211-023-00491-6].

  2023
  • Kaoru Hiramoto, Keika Komatsu, Ryota Shikuwa, An Konno, Yusuke Sato, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku
    "Evaluation of respiratory and secretory activities of multicellular spheroids via electrochemiluminescence imaging"
    Electrochim. Acta., 458, 142507(2023). [DOI:10.1016/j.electacta.2023.142507].

  • Yusuke Sato, Yoshihide Takaku, Toshiaki Nakano, Ken Akamatsu, Dai Inamura, Seiichi Nishizawa
    "Synthetic DNA binders for fluorescence sensing of thymine glycol-containing DNA duplexes and inhibition of endonuclease activity"
    Chem. Commun., 59, 6088-6091 (2023). [DOI:10.1039/d3cc01501g].

  • Chioma Uche Okeke, Hiromasa Miura, Yusuke Sato, Seiichi Nishizawa
    "Kinetic analysis for highly effective triplex formation between small molecule-peptide nucleic acid conjugate probe and Influenza A virus RNA promoter region at neutral pH"
    Org. Biomol. Chem., 21, 3402-3410 (2023). [DOI:10.1039/D3OB00262D].

  • Ryoya Sekine, Satsuki Tsuno, Hayato Irokawa, Kazuhiro Sumitomo, Tianxue Han, Yusuke Sato, Seiichi Nishizawa, Koki Takeda and Shusuke Kuge
    "Inhibition of SARS-CoV-2 nucleocapsid protein–RNA interaction by guanosine oligomeric RNA"
    J. Biochem., 173, 447-457(2023) [DOI:10.1093/jb/mvad008].

  • Kaito Ohira, Yusuke Sato, Seiichi Nishizawa
    "Self-Assembly and Disassembly of Membrane Curvature-Sensing Peptide-Based Deep-Red Fluorescent Probe for Highly Sensitive Sensing of Exosomes"
    ACS Sens., 8, 522-526 (2023). [DOI:10.1021/acssensors.2c02498]

  • Michiyuki Suzuki, Yusuke Sato, Nao Togashi, Seiichi Nishizawa
    "Cationic oligopeptides with amino groups as synthetic nuclear localization signal (NoLS) for the rational design of nucleolus-staining probes"
    ACS Omega, 8, 9592−9596 (2023). [DOI:10.1021/acsomega.3c00116]

  • Mengmeng He, Yusuke Sato, Seiichi Nishizawa
    "Classical thiazole orange and its regioisomer as fluorogenic probes for nucleolar RNA imaging in living cells"
    Analyst, 148, 636-642 (2023). [DOI:10.1039/D2AN01804G]

  2022
  • Takeshi Uemura, Shunsuke Fujii, Hiroki Yamazaki, Tetsuji Itoh, Kouji Masumoto, Seiichi Nishizawa
    "Development of Small-sized Lysine Enzyme Sensor for Clinical Use"
    Sensors and Materials, 34, 3173-3183 (2022). [DOI:10.18494/SAM3914]


  • Takeshi Uemura, Hiroki Yamazaki, Tetsuji Itoh, Seiichi Nishizawa
    "Enzyme Sensor for Simultaneous Determination of Cholesterol, Triglyceride and HDL Cholesterol in Serum"
    Anal. Sci., 38, 1189-1197 (2022). [DOI: 10.1007/s44211-022-00148-w] (Selected as "Hot Article 2022")

  • Kei Higuchi, Yusuke Sato, Nao Togashi, Michiyuki Suzuki, Yukina Yoshino, Seiichi Nishizawa
    "Bright and light-up sensing of benzo[c,d]indole-oxazolopyridine cyanine dye for RNA and its application to highly sensitive imaging of nucleolar RNA in living cells"
    ACS Omega,7, 23744-23748 (2022). [DOI:10.1021/acsomega.2c02408](Highlighted at the Cover)

  • Yusuke Sato, Hiromasa Miura, Takaaki Tanabe, Chioma Uche Okeke, Akiko Kikuchi, Seiichi Nishizawa
    "Fluorescence sensing of the panhandle structure of the influenza A virus RNA promoter by thiazole orange base surrogate-carrying PNA conjugated with small molecule"
    Anal. Chem., 94, 7814-7822(2022). [DOI:10.1021/acs.analchem.1c05488](Highlighted at the Supplementary Cover Art)

  • 西澤精一, 佐藤貴哉, LEE En Ting Tabitha, 坂本直柔, 千葉年輝, 田邉貴昭, 芳野幸奈, 高橋勇樹, 佐藤雄介
    "シアニン色素擬塩基を有する三重鎖形成ペプチド核酸プローブによるRNA二重鎖構造蛍光検出"
    分析化学, 71, 133-144(2022). [DOI:10.2116/bunsekikagaku.71.133] （総合論文：分析試薬研究懇談会特集号）

  2021
  • 西澤精一, LEE En Ting Tabitha, 芳野幸奈, 矢島さやか, 六川正文, 佐藤雄介
    "rRNAを標的とする蛍光プローブの分子設計：FID法における蛍光インジケータと生細胞核小体イメージング蛍光色素"
    分析化学, 70, 703-714 (2021). [DOI:10.2116/bunsekikagaku.70.703]（総合論文：「第81回分析化学討論会」特集号）

  • Yusuke Sato, Yugo Igarashi, Michiyuki Suzuki, Kei Higuchi, Seiichi Nishizawa
    "Deep-red fluorogenic cyanine dyes carrying amino group-terminated side chain for the improved RNA detection and nucleolar RNA imaging"
    RSC Adv., 11, 35436-35439 (2021). [DOI:10.1039/d1ra05872j]

  • Takaya Sato, Yusuke Sato, Seiichi Nishizawa
    "Spectroscopic, Thermodynamic and Kinetic Analysis of Selective Triplex Formation by Peptide Nucleic Acid with Double-stranded RNA over its DNA Counterpart"
    Biopolymers, e23474 (2021). [DOI:10.1002/bip.23474]

  • 伊東良子, 佐藤雄介, 寺前紀夫, 西澤精一
    "HIV-1 TAR RNAを標的とする蛍光インジケータ：モノメチンシアニン色素チアゾールオレンジの機能評価とFIDアッセイへの適用"
    分析化学, 70, 149-157 (2021). [DOI:10.2116/bunsekikagaku.70.149]（化学センサー研究懇談会特集号）

  2020
  • En Ting Tabitha Lee, Yusuke Sato, Seiichi Nishizawa
    "Small Molecule-PNA Oligomer Conjugates for rRNA A-site at neutral pH for FID Assays"
    Chem. Commun., 56, 14976-14979 (2020). [DOI:10.1039/D0CC06084D] (Highlighted at Front Cover)

  • Yusuke Sato, Kazuki Kuwahara, Kenta Mogami, Kenta Takahashi, Seiichi Nishizawa
    "Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing"
    RSC Advances, 10, 38323-38327 (2020). [DOI:10.1039/d0ra07763a]

  • Chun-xia Wang, Yusuke Sato, Takashi Sugimoto, Norio Teramae, and Seiichi Nishizawa
    "Chloro-substituted naphthyridine derivative and its conjugate with thiazole orange for highly selective fluorescence sensing of an orphan cytosine in the AP site-containing duplexes"
    Applied Sciences, 10, 4133(2020). [DOI:10.3390/app10124133]
    (Invited to Special Issue "Nucleic Acids Conjugates for Biotechnological Applications")

  • Yusuke Sato, Yuki Takahashi, Takaaki Tanabe and Seiichi Nishizawa
    "Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA"
    Org. Biomol. Chem., 18, 4009-4013(2020). [DOI:10.1039/d0ob00794c]

  • Yusuke Sato
    "Design of fluorescent peptide nucleic acid probes carrying cyanine dyes for targeting double-stranded RNAs for analytical applications"
    Bull. Chem. Soc. Jpn., 93, 406-413(2020). [DOI:10.1246/bcsj.20190361]

  2019
  • Y. Yoshino, Y. Sato, S. Nishizawa
    "Deep-red light-up signaling of benzo[c,d]indole-quinoline monomethine cyanine for imaging of nucleolar RNA in living cells and for sequence-selective RNA analysis"
    Anal. Chem., 91, 14254-14260 (2019). [DOI:10.1021/acs.analchem.9b01997] (Highlighted at the Supplementary Cover Art)

  • Y. Sato, Y. Aiba, S. Yajima, T. Tanabe, K. Higuchi, S. Nishizawa
    "Strong binding and off-on signaling functions of deep-red fluorescent TO-PRO-3 for influenza A virus RNA promoter region"
    ChemBioChem., 20, 2752-2756(2019). [DOI:10.1002/cbic.201900331] (Highlighted at Cover Feature)

  • Y. Furuhata, M. Kobayashi, R. Maruyama, Y. Sato, K. Makino, T. Michiue, H. Yui, S. Nishizawa, K. Yoshimoto
    "Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation"
    RNA, 25, 590-599(2019).[DOI:10.1261/rna.069062.118]

  • Y. Sato, S. Yajima, A. Taguchi, K. Baba, M. Nakagomi, Y. Aiba, S. Nishizawa
    "Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA"
    Chemical Communications, 55, 3183 - 3186(2019). [DOI:10.1039/C9CC00414A] (Highlighted at Front Cover)

  2018
  • T. Tanabe, T. Sato, Y. Sato, S. Nishizawa
    "Design of a fluorogenic PNA probe capable of simultaneous recognition of 3’-overhang and double-stranded sequences of small interfering RNAs"
    RSC Advances, 8, 42095-42099(2018). [DOI:10.1039/C8RA08759H]

  • Y. Sato, M. Kaneko, T. Sato, S. Nakata, Y. Takahashi, S. Nishizawa
    "Enhanced binding affinity of siRNA overhang-binding fluorescent probes by conjugation with cationic oligopeptides for the improved analysis of siRNA delivery process"
    ChemBioChem., 20, 408-414(2019). [DOI:10.1002/cbic.201800560]

  • Y. Sato, M. Rokugawa, S. Ito, S. Yajima, H. Sugawara, N. Teramae, and S. Nishizawa
    "Fluorescent trimethylated naphthyridine derivative with an aminoalkyl side chain as the tightest non-aminoglycoside ligand for the bacterial A-site RNA"
    Chem. Eur. J., 24, 13862-13870(2018). [DOI: 10.1002/chem.201802320]
    [プレスリリース（東北大学大学院理学研究科HP）]

  • 佐藤雄介, 佐藤貴哉, 西澤精一
    "RNA結合性蛍光プローブの設計・合成と小分子RNA解析"
    分析化学, 67, 531-540(2018).（総合論文） [DOI: 10.2116/bunsekikagaku.67.531]

  • T. Masuda, Y. Shibuya, S. Arai, S. Kobayashi, S. Suzuki, J. Kijima, T. Itoh, Y. Sato, S. Nishizawa, A. Yamaguchi
    "Effect of Cavity Size of Mesoporous Silica on Short-DNA Duplex Stability"
    Langmuir, 34, 5545-5550(2018). [DOI: 10.1021/acs.langmuir.8b00437]

  • T. Sato, N. Sakamoto, S. Nishizawa
    "Kinetic and thermodynamic analysis of triplex formation between peptide nucleic acid and double-stranded RNA"
    Org. Biomol. Chem., 16, 1178-1187(2018). [DOI: 10.1039/C7OB02912H]

  • Y. Sato, D. Iwasawa, P. H. Kuo, R. Nakagomi, S. Nishizawa
    "Improved boronate affinity electrophoresis by optimization of the running buffer for a single step separation of piRNA from mouse testis total RNA"
    Anal. Sci., 34, 627-630(2018). [DOI : 10.2116/analsci.17N024]

  2017
  • T. Chiba, T. Sato, Y. Sato, S. Nishizawa
    "Red-emissive triplex-forming PNA probes carrying cyanine base surrogates for fluorescence sensing of double-stranded RNA"
    Org . Biomol. Chem., 15, 7765-7769 (2017). [DOI: 10.1039/C7OB02077E]

  • T. Sato, Y. Sato, S. Nishizawa
    "Optimization of the Alkyl Linker of TO Base Surrogate in Triplex-Forming PNA for Enhanced Binding to Double-stranded RNA"
    Chem. Eur. J., 23, 4079–4088 (2017). [DOI:10.1002/chem.201604676] (Highlighted on the ChemistryViews website, Selected as Hot Paper, Highlighted at Inside Back Cover)

  2016
  • Y. Sato, H. Saito, D. Aoki, N. Teramae, S. Nishizawa
    "Lysine linkage in abasic site-binding ligand–thiazole orange conjugates for improved binding affinity to orphan nucleobases in DNA/RNA hybrids "
    Chem. Commun., 52, 14446-14449 (2016). [DOI: 10.1039/C6CC07236D]

  • T. Sato, Y. Sato, S. Nishizawa
    "Triplex-forming Peptide Nucleic Acid Having Thiazole Orange as a Base Surrogate for Fluorescence Sensing of Double-stranded RNA "
      J. Am. Chem. Soc., 138, 9397-9400 (2016). [DOI: 10.1021/jacs.6b05554]

  • T. Suzuki, Y. Shibuya, T. Sato, S. Nishizawa, I. Sato, A. Yamaguchi
    "Thermodynamics of Complexation between Thiourea-based Receptor and Acetate in Water/Acetonitrile Mixture"
      Anal. Sci., 32, 741-744 (2016). [DOI: 10.2116/analsci.32.741 ]

  • Y. Sato, T. Asami, Y. Toriyabe, T. Sato, N. Teramae, S. Nishizawa
    "Pteridine Derivatives Modified with a Guanidine for Binding and Sensing an Orphan Guanine in RNA duplexes "
      Chem. Lett., 45, 982-984 (2016). [DOI: 10.1246/cl.160425]

  2015
  • T. Sato, Y. Sato, K. Iwai, S. Kuge, N. Teramae, S. Nishizawa
    "Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe"
      Anal. Sci., 31, 315-320 (2015). [DOI: 10.2116/analsci.31.315]

  • T. Sato, Y. Sato, K. Iwai, S. Kuge, S. Nishizawa, N. Teramae
    "Synthetic fluorescent probes capable of selective recognition of 3'-overhanging nucleotides for siRNA delivery imaging"
      Chem. Commun., 51, 1421-1424 (2015). [DOI: 10.1039/C4CC08800J](Back Cover)

  • J. Tao, P. Song, Y. Sato, S. Nishizawa, N. Teramae, A. Tong, Y. Xiang
    "A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity"
      Chem. Commun., 51, 929-932 (2015). [DOI: 10.1039/C4CC06170E]

  2014
  • Y. Sato, T. Sato, T. Sato, S. Nishizawa, N. Teramae
    "Effect of LNA nucleobases as an enhancer for the binding of amiloride to an abasic site in DNA/DNA and DNA/RNA duplexes"
      Org. Biomol. Chem., 12, 7250-7256 (2014). [DOI: 10.1039/C4OB00977K]

  • Y. Sato, M. Kudo, Y. Toriyabe, S. Kuchitsu, C.-X. Wang, S. Nishizawa, N. Teramae
    "Abasic Site-Binding Ligands Conjugated with Cyanine Dyes for "Off-On" Fluorescence Sensing of Orphan Nucleobases in DNA Duplexes and DNA/RNA Hybrids"
      Chem. Commun., 50, 515-517 (2014). [DOI: 10.1039/C3CC47717G](Front Cover)


  • S. Nishizawa, Y. Sato, N. Teramae
    "Recent Progress in Abasic Site-binding Small Molecules for Detecting Single-base Mutations in DNA"
      Anal. Sci., 30, 137-142 (2014). [DOI: 10.2116/analsci.30.137](Invited review)

  2013
  • Y. Sato, Y. Toriyabe, S. Nishizawa, N. Teramae
    "2,4-Diamino-6,7-dimethylpteridine as a Fluorescent Ligand for Binding and Sensing an Orphan Cytosine in RNA Duplexes"
      Chem. Commun., 49, 9983-9985 (2013). [DOI: 10.1039/C3CC46085A]

  • A. Futamura, A. Uemura, T. Imoto, Y. Kitamura, H. Matsuura, C.-X. Wang, T. Ichihashi, Y. Sato, N. Teramae, S. Nishizawa, T. Ihara
    "Rational Design for Cooperative Recognition of Specific Nucleobases Using β-Cyclodextrin-Modified DNAs and Fluorescent Ligands on DNA and RNA Scaffolds"
      Chem. Eur. J., 19, 10526-10535 (2013). [DOI: 10.1002/chem.201300985]

  • Y. Sato, T. Kageyama, S. Nishizawa, N. Teramae
    "Competitive binding of abasic site-binding ligands and masking ligands to DNA duplexes for the analysis of single base mutation"
      Anal. Sci., 29, 15-19 (2013). [DOI: 10.2116/analsci.29.15]

  Review Article

  Book
  • (応用編　第8章　生体成分　1. 核酸分析　2. 核酸増幅法　西澤精一）
    先端の分析法　第２版
    (株)エヌ・ティー・エス（2022） [ISBN 978-4-86043-737-4 C3058]パンフレット

  • (第4章 細胞外小胞研究の新技術 8.エクソソームを標的とする分子プローブの開発 佐藤雄介）
    実験医学増刊 Vol.39 No.20 EVs 細胞外小胞の生物学
    羊土社(2021)[ISBN 978-4-7581-0399-2]

  • 本水昌二・朝本紘充・石坂昌司・井原敏博・内山一美・齊藤和憲・佐藤健二 塚原　聡・中釜達朗・西澤精一・沼田　靖・南澤宏明・森田孝節・吉川賢治
    基礎教育シリーズ　分析化学　機器分析編（第２版）
    東京教学社（2021）[ISBN 978-4-8082-3059-3]

  • 本水昌二・朝本紘充・石坂昌司・井原敏博・内山一美・齊藤和憲・佐藤健二 塚原　聡・中釜達朗・西澤精一・沼田　靖・南澤宏明・森田孝節・吉川賢治
    基礎教育シリーズ　分析化学　基礎編（第２版）
    東京教学社（2021）[ISBN 978-4-8082-3058-6]

  • [監修] 日本核酸化学会　[編] 杉本直己
    核酸科学ハンドブック
    講談社（2020）[ISBN: 978-4-06-520786-4]
    （第II部　核酸科学の最前線　7. 非ワトソンークリック塩基対結合分子　西澤精一）

  • G. D. Christian, P. K. Dasgupta, K. A. Schug 著 今任稔彦・角田欣一監訳（分担翻訳）
    クリスチャン分析化学　原書7版　II. 機器分析編
    丸善出版（2017）[ISBN: 978-4-621-30110-4]

  • G. D. Christian, P. K. Dasgupta, K. A. Schug 著 今任稔彦・角田欣一監訳（分担翻訳）
    クリスチャン分析化学　原書7版　I. 基礎編
    丸善出版（2016）[ISBN: 978-4-621-30109-8]

  Others
  • 西澤精一
    「三重鎖形成ペプチド核酸（PNA）によるウイルスRNA（vRNA）検出」
    化学と工業, Vol. 75-9 September 2022, 656. （Division Topics, 分析化学ディビジョン）
  • 西澤精一
    「コロナ禍と分析化学」
    ぶんせき, 2022, 173（第5号　とびら）
  • 西澤精一、佐藤雄介
    「RNA解析・生細胞イメージングのための深赤色蛍光色素」
    ぶんせき, 2020, 450-454.
    （第11号、特集　生命現象の”見える化"を支える分析化学）
  • リー エン ティン タビサ
    「グラフェン酸化物を利用した抗HIV薬剤の探索」
    ぶんせき, 2020 第4号 トピックス, 132.
  • 佐藤貴哉, 佐藤雄介, 西澤精一
    「RNA解析・イメージングのための蛍光性核酸プローブの分子設計」
    ぶんせき, 2017, 459-463.
  • 佐藤貴哉, 佐藤雄介, 西澤精一
    「RNA二重鎖を読み解く新技術－三重鎖核酸形成を利用する蛍光プローブ」
    月刊「化学」72巻4月号, 47-52



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